Highly Reproducible Sample Preparation for LC/MS: New Application Note

固相萃取有哪些缺陷?

作为一种样品制备方法，固相萃取被广泛认为是一种“去”的工具，用于在使用联用技术(如LC/MS或GC/MS)分析前清理样品。固相萃取为分析人员提供了几个优势，包括更少的系统停机时间和故障排除，更清洁的色谱，减少污染化合物，以及更大的分析物的可重复性回收率。不幸的是，传统的SPE方法依赖于松散填充的树脂，这往往会造成吸附床上的空隙导致溶液流动不一致等问题。这些不良影响导致分析物和活性树脂之间的相互作用减少，导致结果不一致和分析物回收率较差。

Solution for robust and reproducible LC/MS Sample Prep

An in-depth application report from Porvair Sciences demonstrates how their Microlute® CP Reverse Phase Solid Phase Extraction (SPE) microplates provide a robust and highly reproducible SPE LC-MS sample preparation methodology for a range of neutral, acidic, and basic analytes.

Designed to overcome these shortfalls, Microlute® CP SPE microplates from Porvair Sciences, incorporate a unique, polymer structure made up of an interconnected network of evenly distributed pores combined with retentive media. This hybrid technology ensures even liquid flow rates throughout the SPE process, which leads to sufficient time for Van der Waals forces of interaction to take place between the solid phase sorbent and the analytes resulting in a highly reproducible SPE method.

Experimental data is provided in the application report to show how Microlute® CP RP 96 well microplates selectively retain and elute a wide range of neutral, basic and acidic compounds. The data demonstrate how percentage relative standard deviation values from the Microlute® CP are on average significantly lower than using other commercial SPE plates, thereby producing more reproducible results. In addition, the application notedemonstrates that no analyte is lost in the load step of the SPE process leading to high recovery values for all types of compounds. The Microlute® CP RP 96 well-plate for solid phase extractions is also shown to offer significant benefits for the recovery of hydrophobic basic analytes.

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