Automating Nucleic Acid Purification Kit Protocols

Integra Biosciences has collaborated closely with nucleic acid extraction experts Macherey-Nagel (MN) to develop automated protocols for a variety of NucleoSpin® RNA and DNA extraction and purification kits on theASSIST PLUS pipetting robot. The combination of these technologies provides high quality, purity and yield for DNA and RNA extracts from varied specimen types, suitable for use in a range of applications.

Each MN kit comes in a 96 well format, and can be easily automated on the ASSIST PLUS using a 12 channel 1250 μl VIAFLO electronic pipette with1250 μl Sterile, Filter GripTips. Preset, biologically validated protocols can be quickly downloaded through VIALAB software, offering walk-away pipetting and incubation for:

MN NucleoSpin 96 RNA extraction kit - for extracting and purifying RNA fragments (>200 nt) from cell cultures, tissues and saliva in high throughput.

MN NucleoSpin 96 Tissue kit - for high throughput extraction and purification of high molecular weight genomic DNA from animal tissue samples or animal/bacterial cells.

MN NucleoSpin 96 Plasmid Kit - for parallel extraction and purification of high-copy plasmid DNA with a vector size of less than 25 kbp.

MN NucleoSpin 96 Plasmid Transfection-grade kit - for high throughput extraction and purification of high-copy, transfection-grade E. coli plasmid DNA with a vector size <25 kbp.

Automating liquid handling tasks using the ASSIST PLUS eliminates ergonomic hazards and provides more hands-free time for the user. Pipetting errors and variability between users are avoided, and the validated workflows consistency guarantee maximum reproducibility. In addition, the flexibility of this system ensures that it will always have a purpose in the lab, and can be used to simplify a wide range of general life sciences and diagnostics tasks for far-reaching applications.

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