New Light Sheet Fluorescence Microscope Introduced

The high stability of Zeiss Lightsheet 7 enables researchers to observe living samples over extended periods of time - even days - with less phototoxicity than ever before. The new light sheet microscope can also be used to image very large optically cleared specimens in toto, and with subcellular resolution. The dedicated optics, sample chambers, and sample holders of Zeiss Lightsheet 7 can be adjusted to the refractive index of the chosen clearing method to observe large samples, even whole mouse brains.

Zeiss Lightsheet 7 is designed to match a multitude of conditions. Specimens with a size up to 2 cm, with a refractive index between 1.33 and 1.58, and in almost any clearing solution can be accommodated. The stable turnkey system allows acquisition of overview images as well as of data with subcellular resolution. Researchers can now perform fast and gentle LSFM imaging of optically cleared organoids, spheroids, organs, brains, or other specimens.

蔡司Lightsheet 7具有高量子效率的pco特性。边缘sCMOS探测器能够在最低光照水平下观测最快的过程。科学家们能够获得大样本的真实视图，而不受激发光对其生物学的不利影响。对于垂直方向的样品和最高帧率，可以使用CMOS探测器蔡司Axiocam 702。一个特殊的样品室提供加热，冷却和二氧化碳，以保持最佳环境的活细胞实验。添加Multiview和触发选项来控制外部设备，使得在几乎无限范围的生物体中观察活体过程成为可能。

Zeiss Lightsheet 7 brings LSFM imaging a step further to tackle a broad range of applications with best image quality. Newly designed optics and sample chambers allow users to adjust the optics to the refractive index of their samples. The new sample holder makes mounting larger specimens easier. Smart software tools assist in defining imaging parameters, such as light sheet and sample positions, the right zoom settings, tiles and positions as well as data processing parameters. All these new features go hand in hand with the reliable Zeiss combination of cylindrical lens optics and laser scanning to generate the illumination light sheet. The patented Pivot Scan technology delivers artifact-free optical sections for best possible image quality.